In 2019, stress hormones from tiger scat samples in Bandhavgarh and Kanha Tiger Reserves in Madhya Pradesh revealed that big cats are highly stressed during the tourist season compared with the non-tourism period in both reserves. In a previous study by the authors, published in 2015, showed that tigers introduced in Sariska Tiger Reserve in Rajasthan failed to reproduce, probably due to stress elicited by human disturbances. over-expression of the stress biomarker Cortisol can have life-long significant negative impact on all aspects of wildlife ecology including growth and development, maturation, reproductive fitness, behaviour and survival. The Supreme Court in 2012 extended the ban on tourists in core zones of India’s tiger reserves to prevent misuse wildlife tourism.